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Literature summary for 3.4.24.84 extracted from

  • Clark, K.M.; Jenkins, J.L.; Fedoriw, N.; Dumont, M.E.
    Human CaaX protease ZMPSTE24 expressed in yeast Structure and inhibition by HIV protease inhibitors (2017), Protein Sci., 26, 242-257 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.84

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain BJ5460 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 24% (w/v) PEG 3350, 170 mM ammonium sulfate, 15% (v/v) glycerol Homo sapiens

Protein Variants

Protein Variants Comment Organism
L94P the mutation severely impairs enzyme activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
atazanavir
-
 Homo sapiens
darunavir
-
 Homo sapiens
lopinavir
-
 Homo sapiens
ritonavir
-
 Homo sapiens
tipranavir
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a-factor-CaaX + H2O Homo sapiens
-
 a-factor-C + aaX
-
 ?
METP-(639aa)-RSYLLGNSSPRTQSPQNC(farnesyl)-OCH3 + H2O Homo sapiens
-
 METP-(639aa)-RSY + LLGNSSPRTQSPQNC(farnesyl)-OCH3
-
 ?
prelamin A + H2O Homo sapiens
-
 lamin A + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O75844
-
-

Purification (Commentary)

Purification (Comment) Organism
IgG Sepharose 6 column chromatography, nickel affinity resin column chromatography, and Superdex S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a-factor-CaaX + H2O
-
 Homo sapiens a-factor-C + aaX
-
 ?
Abz-K-SKTKC(farnesyl)VI(-dinitrophenyl derivative of lysine) + H2O
-
 Homo sapiens ?
-
 ?
METP-(639aa)-RSYLLGNSSPRTQSPQNC(farnesyl)-OCH3 + H2O
-
 Homo sapiens METP-(639aa)-RSY + LLGNSSPRTQSPQNC(farnesyl)-OCH3
-
 ?
prelamin A + H2O
-
 Homo sapiens lamin A + ?
-
 ?

Synonyms

Synonyms Comment Organism
Zmpste24
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0022
-
 tipranavir at pH 7.5 and 25°C Homo sapiens
0.0056
-
 lopinavir at pH 7.5 and 25°C Homo sapiens
0.015
-
 ritonavir at pH 7.5 and 25°C Homo sapiens
0.049
-
 atazanavir at pH 7.5 and 25°C Homo sapiens
0.05
-
 darunavir Ki above 0.05 mM, at pH 7.5 and 25°C Homo sapiens